Recombinant preparation of selected bromelain fractions

C - Chemistry – Metallurgy – 12 – N

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C12N 9/50 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01) C12N 15/57 (2006.01) A23J 3/34 (2006.01) A61K 38/48 (2006.01) A61Q 19/00 (2006.01)

Patent

CA 2699429

The present invention pertains in general to Bromelain and particularly to the different active compounds contained in this complex mixture of proteins. The present invention provides recombinant expressed cysteine proteases, which are found in Bromelain. It has been found that the method for expression of the recombinant proteins is superior to the purification from Bromelain itself.

La présente invention porte, d'une manière générale, sur la bromelaïne et, en particulier, sur les différents composés actifs contenus dans ce mélange complexe de protéines. La présente invention porte sur des cystéine protéases exprimées recombinantes, trouvées dans la bromelaïne. Il a été découvert que le procédé d'expression des protéines recombinantes est supérieur au procédé de purification de la bromelaïne elle-même.

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