Recombinant production of antimicrobial agents

Details Recombinant production of antimicrobial agents Recombinant production of antimicrobial agents

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01K 67/027 (2006.01) A23K 1/00 (2006.01) A23K 1/17 (2006.01) C07K 1/107 (2006.01) C07K 14/47 (2006.01) C12N 9/90 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2536782

The invention relates to the use of co-expression of an antimicrobial agent and an enzyme, with a view to improving yield and/or overall production economy. Examples of antimicrobial agents are antimicrobial peptides such as lactoferricins and antimicrobial enzymes such as lysozyme and glucose oxidase, and examples of enzymes are endoglucanase, xylanase, phytase, protease, galactanase, mannanase, dextranase, alpha-galactosidase, pectate lyase, alpha- amylase and glucoamylase. A fusion product comprising the antimicrobial agent and the enzyme and a cleavable linker is novel, and can be used in animal feed and animal feed additives. The invention also describes the use of a protection domain wherein at least 50% of the amino acid residues comprised in the peptide protection domain are D (Asp) and/or E (Glu). The protection or quenching domain serves to temporarily and reversibly inactivate the antimicrobial peptide during the expression.

L'invention concerne l'utilisation de la co-expression d'un agent antimicrobien et d'une enzyme, dans le but d'améliorer le rendement et l'économie de production globale. Les agents antimicrobiens cités à titre d'exemple sont les peptides antimicrobiens, tels que les lactoferricines, et les enzymes antimicrobiennes, telles que le lysozyme et la glucose-oxydase, et les enzymes peuvent être, entre autres, l'endoglucanase, la xylanase, la phytase, la protéase, la galactanase, la mannanase, la dextranase, l'alpha-galactosidase, la pectate-lyase, l'alpha-amylase et la glucoamylase. Un produit de fusion comprenant l'agent antimicrobien et l'enzyme ainsi qu'un lieur est nouveau et peut être utilisé dans des aliments pour animaux et dans des additifs alimentaires pour animaux. L'invention porte également sur l'utilisation d'un domaine de protection, dans lequel au moins 50 % des résidus d'acide aminé compris dans le domaine de protection du peptide sont D (asp) et/ou E (Glu). Le domaine de protection ou d'extinction sert à inactiver temporairement et de manière réversible le peptide antimicrobien pendant l'expression.

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