Recombinant production of toxoplasma sag1 antigen

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/30 (2006.01) A61K 39/002 (2006.01) C07K 14/45 (2006.01) C12N 15/81 (2006.01) G01N 33/569 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2330209

The invention provides a method for the production of the toxoplasma antigen SAG1 or a fragment thereof, which comprises: (a) constructing a plasmid comprising DNA encoding SAG1 or a fragment thereof; (b) transforming a P. pastoris host cell with the said plasmid; and culturing the host such that the DNA encoding SAG1 or a fragment thereof is expressed. The invention further provides truncated SAG1 proteins in which the anchor region is absent, particularly truncated SAG1 comprising amino acid residues (48-307) of SAG1, and vaccine compositions comprising the SAG1.

L'invention concerne un procédé de production de l'antigène de toxoplasme SAG1 ou d'un fragment de celui-ci, qui comporte les étapes consistant à: (a) construire un plasmide comportant l'ADN codant pour SAG1 ou pour un fragment de celui-ci ; (b) transformer une cellule hôte de P. pastoris à l'aide dudit plasmide ; et cultiver l'hôte de façon à exprimer l'ADN codant pour SAG1 ou pour un fragment de celui-ci. L'invention a en outre trait à des protéines de SAG1 tronqué dans lesquelles la région d'ancrage est absente, notamment de SAG1 tronqué comportant les résidus d'acides aminés 48-307 de SAG1, et à des compositions de vaccin renfermant SAG1.

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