Recombinant secoisolariciresinol dehydrogenase, and methods...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/00 (2006.01) C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/04 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12N 15/29 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2326380

A secoisolariciresinol dehydrogenase protein has been isolated from Forsythia intermedia, together with cDNAs encoding secoisolariciresinol dehydrogenase from this species. Accordingly, isolated DNA sequences are provided which code for the expression of secoisolariciresinol dehydrogenase. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a secoisolariciresinol dehydrogenase protein, or to a base sequence sufficiently complementary to at least a portion of a secoisolariciresinol dehydrogenase DNA or RNA to enable hybridization therewith. Thus, systems and methods are provided for the recombinant expression of secoisolariciresinol dehydrogenases that may be used to facilitate the production, isolation and purification of significant quantities of recombinant secoisolariciresinol dehydrogenase for subsequent use, to obtain expression or enhanced expression of secoisolariciresinol dehydrogenase in plants in order to enhance, or otherwise alter, lignan biosynthesis, or may be otherwise employed for the regulation or expression of secoisolariciresinol dehydrogenase.

Une protéine, la secoisolaricirésinol déshydrogénase, a été isolée à partir de Forsythia intermedia, en même temps que des ADNc codant pour la secoisolaricirésinol déshydrogénase de cette espèce. L'invention concerne donc des séquences d'ADN qui codent pour l'expression de la secoisolaricirésinol déshydrogénase. Dans d'autres aspects, elle concerne des vecteurs de clonage recombinants réplicables, qui renferment une séquence d'acide nucléique codant pour une secoisolaricirésinol déshydrogénase, ainsi qu'une séquence de bases suffisamment complémentaire d'au moins une partie d'un ADN ou d'un ARN de secoisolaricirésinol déshydrogénase pour pouvoir s'hybrider avec elle. L'invention concerne également des systèmes et des méthodes permettant l'expression recombinante de secoisolaricirésinol déshydrogénases, qui peuvent être utilisés pour faciliter la production, l'isolement et la purification de quantités significatives de secoisolaricirésinol déshydrogénase recombinante en vue de son utilisation ultérieure; pour obtenir ou améliorer l'expression de la secoisolaricirésinol déshydrogénase chez les végétaux afin d'améliorer la biosynthèse du lignane ou de la modifier d'une autre manière; ainsi que pour réguler ou exprimer la secoisolaricirésinol déshydrogénase.

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