Recombinant spider silk analogs

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) F02B 75/02 (2006.01)

Patent

CA 2164692

The invention relates to novel spider silk protein analogs derived from the amino acid consensus sequence of repeating units found in the natural spider dragline of Nephila clavipes. More specifically, synthetic spider dragline has been produced from E. coli and Bacillus subtilis recombinant expression systems wherein expressions from E. coli is at levels greater than 1 mg full-length polypeptide per gram of cell mass.

L'invention concerne de nouveaux analogues de protéines de soie d'araignée dérivés de la séquence concensus d'acides aminés d'unités répétitives rencontrées dans le fil d'araignée naturel de Nephila clavipes. Plus spécifiquement, on a produit du fil d'araignée synthétique à partir de systèmes d'expression recombinée à partir de E. coli et de Bacillus subtilis, dans lesquels l'expression à partir de E. coli atteint des niveaux supérieurs à 1 mg de polypeptides de longueur totale par gramme de masse cellulaire.

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