Recombinant staphylococcal nucleases and their use in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/55 (2006.01) A01N 63/00 (2006.01) A61K 35/74 (2006.01) A61K 39/02 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12N 9/22 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12N 15/32 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12S 1/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2173751

The survival of cells can be limited by introducing into the cells a regularly expressible gene whose expression results in the formation of a cytoplasmatically active truncated and/or mutated Staphylococcus aureus nuclease. Populations of cells containing, in addition to such a regulatably expressible gene, a DNA coding for an immunologically active, pesticidally active or environmental pollutant-degrading gene product are useful in immunologically active compositions, pesticidally active compositions and environmental pollutant-degrading compositions, respectively.

Il est possible de limiter la durée de survie de cellules par l'introduction, dans ces cellules, d'un gène dont l'expression, qui peut être régulée, entraîne la formation d'une nucléase de Staphylococcus aureus tronquée et/ou mutée, à activité cytoplasmatique. Les populations de cellules contenant, outre un tel gène dont l'expression peut être régulée, un ADN codant un produit génique à activité immunologique, pesticide ou à dégradation de polluants environnementaux, sont aptes à être utilisées dans des compositions à activité immunologique, des compositions à activité pesticide et des compositions servant à dégrader des polluants environnementaux.

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