Recombinant subunit vaccine

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 2/00 (2006.01) A61K 9/00 (2006.01) A61K 39/12 (2006.01) A61K 39/15 (2006.01) A61K 39/44 (2006.01) A61K 47/42 (2006.01) A61P 37/02 (2006.01) C07K 14/18 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 15/12 (2006.01) C12N 15/866 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2382176

A method is provided of producing an immunogenic complex comprising a heat shock protein (hsp) coupled to a heterologous antigenic polypeptide, which method comprises: (a) expressing the antigenic polypeptide in a cell which cell has been subjected to a stimulus which causes the induction of a heat shock response in said cells; and (b) recovering the antigenic polypeptide coupled to one or more hsps from said cell or the culture medium. Also provided are immunogenic compositions comprising a heat shock protein (hsp) derived from a non-mammalian eukaryote coupled to a heterologous antigenic polypeptide which composition is capable of inducing an immune response to said antigenic polypeptide in a human or animal.

L'invention concerne une méthode de production d'un complexe immunogénique contenant une protéine de stress (hsp) couplée à un polypeptide antigénique hétérologue. Ladite méthode consiste à (a) exprimer le polypeptide antigénique dans une cellule qui a été sujette à un stimulus provoquant l'induction d'une réponse de stress dans ladite cellule, et (b) récupérer le polypeptide antigénique couplé à au moins une hsp de ladite cellule ou du milieu de culture. Cette invention concerne également des compositions immunogéniques contenant une protéine de stress (hsp) dérivée d'un eucaryote non mammalien couplé à un polypeptide antigénique hétérologue. Ladite composition est capable d'induire une réponse immunitaire dans un polypeptide antigénique chez un être humain ou un animal.

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