Recombinant thermostable enzyme which forms non-reducing...

Details Recombinant thermostable enzyme which forms non-reducing... Recombinant thermostable enzyme which forms non-reducing...

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12P 19/12 (2006.01) C12P 19/14 (2006.01)

Patent

CA 2154307

Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 69,000-79,000 daltons and a pI of about 5.4-6.4, and forms non-reducing saccharides having a trehalose structure as an end unit from reducing amylaceous saccharides having a degree of glucose polymerization of at least 3. The enzyme has satisfactorily high thermostability, i.e. it is substantially not inactivated even when incubated in an aqueous solution (pH 7.0) at 85° C for 60 min, and this facilitates the production of such non-reducing saccharides on an industrial scale and in a satisfactorily-high yield.

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