C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12P 19/12 (2006.01) C12P 19/14 (2006.01)
Patent
CA 2154333
Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous 0 solution (pH 7.0) at 85° C for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.
Kubota Michio
Mitsuzumi Hitoshi
Sugimoto Toshiyuki
Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
Macrae & Co.
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