Recombinant trichosanthin and coding sequence

Details Recombinant trichosanthin and coding sequence Recombinant trichosanthin and coding sequence

C - Chemistry, Metallurgy

12

N

C12N 15/29 (2006.01) A61K 38/16 (2006.01) C07K 14/415 (2006.01) C07K 14/73 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2050593

68 ABSTRACT Disclosed are the entire protein and nucleic acid coding sequences for unprocessed and mature trichosanthin protein from Trichosanthes kirilowii. A recombinant tri- chosanthin protein produced from the coding sequence, and the trichosanthin protein with amino-terminal and/or carboxy-terminal extensions are also described. Primers derived from the coding sequence are disclosed for use in obtaining the coding sequences of ribosome-inactivating- proteins which have regions of amino acid sequence identical to those of trichosanthin. Further, a multi- gene family of ribosome-inactivating-protein encoding genes of Trichosanthes kirilowii is also described.

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