C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) C07K 14/01 (2006.01) C07K 14/255 (2006.01) C12N 1/21 (2006.01) C12N 15/34 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01)
Patent
CA 2205074
The present invention relates to recombinant vectors which are useful for directselection of colonies harboring recombinant plasmids, on basis of the loss of expression of a gene coding for an AsiA polypeptide from E. coli bacteriophage T4, the expression of which would normally have resulted in toxicity to the bacterial host. The invention also relates to host cells harboring the said vectors, as well as to methods utilizing the said vectors, for the selection of nucleic acid clones.
Vecteurs recombinants utiles à la sélection directe de colonies abritant des plasmides recombinants en fonction de la perte d'expression d'un gène codant un polypeptide AsiA du bactériophage T4 de E. Coli, dont l'expression aurait normalement dû provoquer un effet toxique pour l'hôte bactérien. L'invention concerne également les cellules hôtes abritant lesdits vecteurs, de même que les méthodes faisant appel aux vecteurs en question pour la sélection de clones d'acide nucléique.
Astra Aktiebolag
Fetherstonhaugh & Co.
LandOfFree
Recombinant vectors does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Recombinant vectors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Recombinant vectors will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1888382