C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/86 (2006.01) A61K 48/00 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12N 15/867 (2006.01) C12N 15/87 (2006.01)
Patent
CA 2145063
We have made retrovirus particles displaying a functional antibody fragment. We fused the gene encoding an antibody fragment directed against a hapten with that encoding the viral envelope protein (Pr80env) of the ecotropic Moloney murine leu- kemia virus. The fusion gene was co-expressed in ecotropic retroviral packaging cells with a retroviral plasmid carrying the neom- ycin phosphotransferase gene (neo), and retroviral particles with specific hapten biding activities were recovered. Furthermore the hapten-binding particles were able to transfer the neo gene and the antibody-envelope fusion gene to mouse fibroblasts. In princi- ple, the display of antibody fragments on the surface of recombinant retroviral particles could be used to target virus to cells for gene delivery, or to retain the virus in target tissues, or for the construction of libraries of viral display packages.
Hawkins Robert Edward
Russell Stephen James
Winter Gregory Paul
Biofocus Discovery Limited
Kirby Eades Gale Baker
LandOfFree
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