Recombinase polymerase amplification

Details Recombinase polymerase amplification Recombinase polymerase amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2650993

The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase 'systems' having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.

L'invention concerne de nouvelles enzymes recombinase diverses, hybrides et produites par génie génétique, et l'utilité de telles protéines avec des facteurs de recombinaison associés pour effectuer des dosages d'amplification d'ADN. L'invention concerne également différents "systèmes" par recombinase présentant des activités biochimiques distinctes dans des dosages d'amplification d'ADN, et des exigences différentes pour des facteurs de charges, des protéines de liaison à l'ADN à simple brin (SSB), et la grandeur de l'agent d'entassement utilisé.

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