Recombinatant mutants of rhabdovirus and methods of use thereof

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/47 (2006.01) A61K 35/76 (2006.01) A61K 48/00 (2006.01) C07K 14/145 (2006.01) C12N 7/01 (2006.01) C12N 15/86 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2498297

The present invention relates to recombinant Rhabdoviridae, isolated nucleic acids, vectors, cells and compositions comprising same. The recombinant Rhabdoviridae, isolated nucleic acids, vectors, cells and compositions express Rhabdoviral proteins including a mutated matrix protein (M) and/or a mutated glycoprotein (G), in addition to expression of at least one foreign nucleic acid. The present invention also relates to methods of use thereof, including their use in vivo, in anti-cancer applications, such as in the treatment of gliomas. The recombinant Rhabdoviridae of the present invention are also useful in gene therapy and vaccine applications.

L'invention concerne des rhabdoviridae recombinés, ainsi que des acides nucléiques isolés, des vecteurs, des cellules et des compositions les contenant. Lesdits rhabdoviridae recombinés, acides nucléiques isolés, vecteurs, cellules et compositions expriment des protéines de rhabdovirus, y compris une protéine matricielle mutée (M) et/ou une glycoprotéine mutée (G), ainsi qu'au moins un acide nucléique étranger. L'invention concerne également des procédés d'utilisation desdits rhabdoviridae recombinés, y compris leur utilisation in vivo, dans des applications pour lutter contre le cancer, telles que le traitement des gliomes. Lesdits rhabdoviridae recombinés sont également utiles dans la thérapie génique et des applications sous forme de vaccins.

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