Recombination cassettes and methods for sequence excision in...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2578140

Summary The invention relates to improved recombination systems and methods for eliminating maker sequences from the genome of plants. Particulary the invention is based on use of an expression cassette comprising the parsley ubiquitin promoter, and operably linked thereto a nucleic acid sequence coding for a sequence specific DNA-endonuclease.

La présente invention concerne des systèmes de recombinaison améliorés et des techniques permettant d'éliminer des séquences de marqueurs du génome de plantes. Cette invention concerne en particulier l'utilisation d'une cassette d'expression comprenant le promoteur d'ubiquitine du persil et, liée de manière opérationnelle, une séquence d'acide nucléique codante pour une endonucléase d'ADN spécifique de séquence.

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