Recombination of nucleic acid library members

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/04 (2006.01) C07K 16/00 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2489595

The invention provides, inter alia, a method of preparing a nucleic acid sequence that encodes a polypeptide that is displayed on a heterologous cell surface. The generally includes recombining a donor nucleic acid and an acceptor nucleic acid to form a recombined nucleic acid that encodes a polypeptide that is displayed. The recombination reaction is typically an in vivo reaction, in that at least the resolution of recombination intermediates occurs within a cell. Both site-specific recombination and homologous recombination can be used.

L'invention concerne, entre autres, un procédé de préparation de séquence nucléotidique codant un polypeptide présenté sur une surface cellulaire hétérologue. En règle générale, le procédé consiste en une recombinaison d'acide nucléique donneur et d'acide nucléique accepteur, pour former un acide nucléique recombiné codant un polypeptide présenté. Généralement, la réaction de recombinaison est une réaction in vivo: en l'occurrence, au moins la résolution des intermédiaires de recombinaison intervient dans une cellule. Il est possible de recourir à la fois à une recombinaison spécifique au site et à une recombinaison homologue.

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