Recombinational cloning using engineered recombination sites

Details Recombinational cloning using engineered recombination sites Recombinational cloning using engineered recombination sites

C - Chemistry, Metallurgy

12

N

C12N 15/63 (2006.01) C12N 15/10 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01) C12N 15/66 (2006.01)

Patent

CA 2226463

Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

Afin de mettre en oeuvre le clonage de recombinaison décrit, on utilise des acides nucléiques, des vecteurs et des procédés in vitro et in vivo pour déplacer ou échanger des segments de molécules d'ADN au moyen de sites et de protéines de recombinaison obtenus par génie génétique, et obtenir des molécules chimériques d'ADN qui ont la ou les caractéristique(s) et/ou le ou les segment(s) d'ADN voulus.

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