Recombinational cloning using nucleic acids having...

Details Recombinational cloning using nucleic acids having... Recombinational cloning using nucleic acids having...

C - Chemistry, Metallurgy

12

N

C12N 15/00 (2006.01) C07H 21/00 (2006.01) C12N 15/64 (2006.01) C12N 15/66 (2006.01) C12P 19/34 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2307016

Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

L'invention concerne un clonage recombinatoire au moyen d'acides nucléiques, de vecteurs et de méthodes, in vitro et in vivo, afin de déplacer et d'échanger des segments de molécules d'ADN par utilisation de sites de recombinaison manipulés et de protéines de recombinaison pour obtenir des molécules d'ADN chimériques possédant les caractéristiques voulues et/ou des segments d'ADN.

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