Recovery and detection of microorganisms from mixed...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/00 (2006.01) C12N 1/10 (2006.01) C12N 1/20 (2006.01) C12Q 1/04 (2006.01) C12Q 1/24 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2739728

The present invention is directed to the recovery of bacteria and microparasites, particularly Cryptosporidium and Giardia, from water samples by filtration through a mixed cellulose ester membrane, partial dissolution of said membrane with methanol followed by completion in the presence of acetone, and purification and concentration using glass beads as a secondary confinement matrix.

La présente invention concerne la récupération de bactéries et de microparasites, en particulier Cryptosporidium et Giardia, à partir d'échantillons d'eau par filtration au travers d'une membrane en ester de cellulose mixte, dissolution partielle de ladite membrane avec du méthanol suivie par la finalisation de la dissolution en présence d'acétone, et purification et concentration en utilisant des billes de verre en tant que matrice de confinement secondaire.

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