Recovery of functional human leukocytes from recycled filters

Details Recovery of functional human leukocytes from recycled filters Recovery of functional human leukocytes from recycled filters

B - Operations, Transporting

01

D

B01D 37/04 (2006.01) A61M 1/36 (2006.01)

Patent

CA 2314150

A method of recovering functional human leukocytes from blood filters used to deplete leukocyte content from leukocyte-containing blood-cell suspensions is provided. More particularly, this method involves isolating leukocytes from filters which have been used to purify red blood cells or platelets in isolation from leukocytes by back-flushing the used filters with a hemolysis solution, such as cold ammonium chloride, and collecting the functional human leukocytes.

La présente invention concerne un procédé de récupération de leucocytes humains fonctionnels à partir de filtres sanguins utilisés pour la déplétion lymphocytaire dans des suspensions de cellules sanguines renfermant des leucocytes. Cette invention consiste plus particulièrement à isoler des leucocytes contenus dans des filtres ayant été utilisés pour purifier les hématies ou les plaquettes sanguines séparées des leucocytes en rinçant à contre-courant les filtres usés avec une solution d'hémolyse, telle que le chlorure d'ammonium froid, et à récupérer ensuite les leucocytes humains fonctionnels.

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