Recovery of virus from cell culture using a hypertonic salt...

Details Recovery of virus from cell culture using a hypertonic salt... Recovery of virus from cell culture using a hypertonic salt...

C - Chemistry, Metallurgy

12

N

C12N 7/02 (2006.01)

Patent

CA 2299628

A process of harvesting a herpesvirus from a cell culture infected therewith comprises treating said culture with a hypertonic aqueous salt solution to yield a virus suspension, e.g. to give improved yield of virus for live virus vaccine where otherwise cell-disruption might be used to harvest the virus by disrupting virus-infected cells. The harvesting step can be followed e.g. by nuclease treatment, diafiltration and lyophilisation.

L'invention concerne un procédé permettant de recueillir un herpèsvirus à partir d'une culture cellulaire infectée par celui-ci, lequel procédé consiste à traiter la culture avec une solution salée aqueuse hypertonique pour obtenir une suspension virale, par exemple afin d'obtenir une meilleure production de virus pour un vaccin à virus vivants. Sinon on peut avoir recours à la rupture de cellules pour recueillir le virus en dissociant les cellules infectées par le virus. L'opération de recueil de virus peut être suivie, par exemple, d'un traitement aux nucléases, d'une diafiltration et d'une lyophilisation.

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