Redox mediator reagent and biosensor

Details Redox mediator reagent and biosensor Redox mediator reagent and biosensor

C - Chemistry, Metallurgy

12

Q

C12Q 1/54 (2006.01) C12M 1/40 (2006.01) C12N 9/04 (2006.01) C12Q 1/00 (2006.01) G01N 27/327 (2006.01)

Patent

CA 2069946

The invention provides an amperometric biosensor for detecting the concentration of an analyte in a fluid sample. The biosensor (1) has a working (4) and a counter electrode (5) fixed to an insulator material (7). A biosensor reagent (11) is placed in a well (9) so that is covers substantially all of the exposed surfaces (10) of the electrodes (4,5). For testing, a sample containing the analyte is added to the reagent well (9), the reaction allowed to go to completion, a potential difference applied to the electrodes (4,5) and the diffusion limited current is measured.

Capteur biologique ampérométrique permettant de détecter la concentration d'un analyte dans un échantillon de fluide. Le capteur biologique (1) comporte une électrode de travail (4) et une contre-électrode (5) fixées à un matériau isolant (7). On place un réactif (11) du capteur biologique dans un puits (9) de manière qu'il couvre sensiblement toutes les surfaces exposées (10) des électrodes (4, 5). Afin d'effectuer la mesure de concentration, on ajoute un échantillon contenant l'analyte au puits de réactif (9), on attend la fin de la réaction, on applique une différence de potentiel aux électrodes (4, 5) et on mesure le courant limité par diffusion.

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