Reduction of non-specific amplification during in vitro...

Details Reduction of non-specific amplification during in vitro... Reduction of non-specific amplification during in vitro...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 15/52 (2006.01) C12Q 1/68 (2006.01) C12S 3/14 (2006.01)

Patent

CA 2087724

Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contam- ination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bases into the amplification reaction products and treating the products by enzy- matic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications.

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