Reflectively coated optical waveguide and fluidics cell...

G - Physics – 01 – N

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Details

G01N 21/77 (2006.01) G01N 21/17 (2006.01) G01N 21/55 (2006.01) G01N 33/543 (2006.01) G02B 6/10 (2006.01) G02B 6/42 (2006.01)

Patent

CA 2366490

A multimode waveguide device for assays includes a multimode waveguide (12) with a patterned, reflective surface coating (22). The exposed portion of the reflectively coated waveguide surface are for analyte recognition. A fluidics cell (10) is attached to the waveguide so that the channel or channels (30) of the fluidics cell (10) match with the exposed portion or portions of the waveguide surface. The channel or channels (30) direct one or more sample fluids over the exposed portion or portions of the waveguide surface. The reflective coating minimizes loss and scattering of excitation and fluorescence light during a fluorescence assay.

L'invention concerne un dispositif de guide d'ondes multimode destiné à des dosages comprenant un guide d'ondes multimode (12) revêtu d'une couche de surface réfléchissante (22) structurée. La partie exposée de la surface du guide d'ondes pourvue d'une couche réfléchissante sert à reconnaître un analyte. Une cellule fluidique (10) est fixée au guide d'ondes, de manière que le ou les canaux (30) de cellules fluidiques (10) coïncident à la ou aux parties exposées de la surface du guide d'ondes. Le ou les canaux (30) dirigent un ou plusieurs fluides de prélèvement sur la ou les parties exposées de la surface du guide d'ondes. La couche réfléchissante réduit au minimum les pertes et la diffusion de lumière d'excitation et de lumière fluorescente lors d'un dosage à fluorescence.

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