Regeneration of a chromatography matrix

B - Operations – Transporting – 01 – J

Patent

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Details

B01J 20/34 (2006.01) B01D 15/08 (2006.01) C07K 1/22 (2006.01)

Patent

CA 2608393

The present invention relates to a process of regenerating a separation matrix, such as a chromatography matrix, comprising adsorption of at least one target molecule by contacting a mobile phase comprising at target molecule(s) with a matrix; removal of unbound material by washing the matrix; elution of target molecule(s) by contacting the matrix with an eluent; reducing regeneration by contacting said matrix with a reducing agent; alkaline regeneration by contacting the matrix with an alkaline solution; and equilibration of the matrix.

L'invention concerne un procédé permettant régénérer une matrice de séparation, par exemple une matrice de chromatographie, qui consiste à adsorber au moins une molécule cible par contact d'une phase mobile comprenant une(des) molécule(s) cible(s) avec une matrice; à évacuer un matériau non relié par lavage de la matrice; à éluer la(les) molécule(s) cible(s) par contact de la matrice avec un éluant; à réduire la régénération par contact de ladite matrice avec un agent réducteur; à réaliser une régénération alcaline par contact de la matrice avec une solution alcaline; et à équilibrer ladite matrice.

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