Regeneration of nad(p) cofactor

C - Chemistry – Metallurgy – 12 – P

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C12P 19/36 (2006.01) C12N 9/04 (2006.01) C12P 1/00 (2006.01) C12P 7/26 (2006.01) C12P 17/04 (2006.01) C12P 17/08 (2006.01) C12P 19/02 (2006.01)

Patent

CA 1207691

ABSTRACT OF THE DISCLOSURE In a process wherein a first substrate is oxidized and NAD(P) is reduced in the presence of a first NAD(P) cofactor-linked oxidoreductase enzyme to form a first reaction product and NAD(P)H2, and subsequently a second substrate and the resulting NAD(P)H2 are reacted in the presence of a second NAD(P) cofactor-linked oxidoreductase enzyme to form a second reaction product and NAD(P), the improvement which comprises reacting said second reaction product with oxygen in the presence of an oxidase enzyme to regenerate said second substrate.

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