Regioselective .alpha.-hydrolysis of amino acid diesters...

C - Chemistry – Metallurgy – 12 – P

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C12P 13/14 (2006.01) C12P 13/04 (2006.01) C12P 13/20 (2006.01)

Patent

CA 2264278

The regioselective and chemoselective hydrolysis of an .alpha.-ester group of an amino acid diester using pig liver esterase enzyme (PLE) is disclosed. The amino acid diesters may be either N-protected or unprotected and the diester groups may be the same or different. In particular, the preparation of a number of .gamma.-ester glutamates and .beta.-ester aspartates are provided.

Selon l'invention, on effectue une hydrolyse régiosélective et chimiosélective d'un groupe .alpha.-ester d'un diester d'acide aminé, à l'aide d'une estérase de foie de porc. Les diesters d'acides aminés peuvent être soit N-protégés, soit non protégés, et les groupes diesters peuvent être semblables ou différents. On décrit notamment la préparation d'un certain nombre de .gamma.-ester glutamates et de .beta.-esters aspartates.

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