Regulating stem cells

Details Regulating stem cells Regulating stem cells

A - Human Necessities

61

K

A61K 38/17 (2006.01)

Patent

CA 2645142

A composition of matter is provided, comprising a population of cultured cells that comprises a sub-population of cells that both stain as CD31Bright and demonstrate uptake of Ac-LDL+, and secrete IL-8. A method is also provided, comprising stimulating in vitro an initiating cell population (ICP) of at least 5 million cells that have a density of less than 1.072 g/ml, wherein at least 1% of the cells of the ICP is CD34+CD45-/Dim, and at least 25% of the cells of the ICP are CD31Bright, to differentiate into a progenitor/precursor cell population (PCP). Other embodiments are also described.

L'invention porte sur une préparation de substances comportant une population de cellules de culture comprenant une sous-population de cellules qui se colorent en tant que CD31Bright et assurent la capture de l'Ac-LDL+, et sécrètent l'IL-8. L'invention porte également sur une méthode consistant à stimuler in vitro une population de cellules initiatrices (ICP) d'au moins 5 millions de cellules d'une densité de moins de 1,072 g/ml et dont au moins 1 % sont des CD34+CD45-/Dim, et au moins 25%, des CD31Bright, pour les différencier en une population de cellules progénitrices et précurseurs. L'invention porte également sur d'autres exécutions.

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