C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/74 (2006.01) C07K 14/21 (2006.01) C12N 1/21 (2006.01) C12N 15/31 (2006.01)
Patent
CA 2619989
Methylotrophic or methanotrophic bacteria such as Methylobacterium are transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator from Pseudomonas putida may serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.
L'invention concerne des bactéries méthylotrophes ou méthanotrophes, par exemple Methylobacterium, qui sont transformées avec un gène d'intérêt, ainsi que la régulation de l'expression de ce gène au moyen d'une protéine répresseur de cumate et une séquence opérateur liée fonctionnellement au gène d'intérêt, et l'addition d'un agent externe. Plus précisément, le répresseur cymR et l'opérateur cmt de Pseudomonas putida peuvent servir à réguler l'expression génique dans les bactéries méthylotrophes ou méthanotrophes avec l'addition de cumate.
Choi Young-Jun
Massie Bernard
Miguez Carlos B.
Mullick Alaka
Cassan Maclean
National Research Council Of Canada
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