Regulation of promoter activity using a polypeptide isolated...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) C07K 14/335 (2006.01) C12N 1/21 (2006.01) C12N 15/67 (2006.01) C12N 15/74 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2426086

Nucleotide sequences coding for a promoter activity-regulating polypeptide designated OrfY, isolated from Lactococcus lactis, and promoter activity- regulating active homologues and fragments hereof is used to indirectly regulating the expression of genes. The coding sequence is inserted into lactic acid bacterial cells or any other cells with the objective of either reducing or enhancing the expression level of the gene being under the control of the promoter sequence, the activity of which is regulated by the OrfY polypeptide or homologues or fragments hereof. Improved gene expression systems are provided based on this promoter activity-regulating polypeptide.

L'invention concerne des séquences nucléotidiques codant un polypeptide de régulation de l'activité du promoteur appelé OrfY, isolées de <i>Lactococcus lactis</i>, et des homologues actifs de régulation de l'activité du promoteur ainsi que des fragments dudit promoteur sont utilisés afin de réguler de manière indirecte l'expression des gènes. La séquence codante est incorporée dans des cellules de bactéries lactiques ou n'importe quelles autres cellules avec l'objectif soit de réduire, soit d'améliorer le niveau d'expression des gènes étant sous le contrôle de la séquence de promoteur, dont l'activité est régulée par le polypetide OrfY, ses homologues ou ses fragments. L'invention concerne enfin des systèmes améliorés d'expression des gènes fondés sur le polypeptide de régulation de l'activité du promoteur.

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