Regulation of viral gene expression

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/11 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2369422

The present invention relates to methods to alter the expression of a viral gene in a cell using sense and antisense RNA fragments of the gene. The sense and antisense RNA fragments are capable of pairing and forming a double- stranded RNA molecule, thereby altering the expression of the gene. The present invention also relates to cells, plants or animals, their progeny and seeds derived thereof, obtained using a method of the present invention. Preferably, such cells, plants or animals are resistant or tolerant to viruses.

L'invention concerne des procédés permettant de modifier l'expression d'un gène viral dans une cellule au moyen de fragments d'ARN sens et antisens dudit gène. Lesdits fragments d'ARN sens et antisens sont capables de s'apparier pour former une molécule d'ARN à double brin et modifier ainsi l'expression du gène. L'invention concerne aussi des cellules, des plantes ou des animaux, leur descendance et des graines dérivés à partir de ceux-ci, qui sont obtenus en utilisant le procédé de la présente invention. De préférence, ces cellules, plantes et animaux sont résistants ou tolérants aux virus.

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