Regulatory region of a lipid transfer protein (ltpw1) from...

Details Regulatory region of a lipid transfer protein (ltpw1) from... Regulatory region of a lipid transfer protein (ltpw1) from...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2387440

This invention is directed to a regulatory re-gion obtained from a wheat aleurone gene LtpW1. This reg-ulatory region, truncated derivatives, mutations, or deletions of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory re-gion that exhibits constitutive activity within both monocot and dicot plants. This invention is also directed to vectors com-prising these regulatory regions operatively linked with a het-erologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this in-vention are also disclosed. Image

L'invention concerne une zone régulatrice obtenue à partir d'une protéine de transfert de lipides (LtpW1) d'un gène d'aleurone. Cette zone régulatrice, les dérivés tronqués, les mutations ou les délétions de ladite zone peuvent être utilisés pour exprimer des gènes hétérologues d'intérêt à l'intérieur des cellules d'aleurone d'une plante. En outre, cette invention concerne une zone régulatrice de LtpW1 tronquée qui présente une activité essentielle au sein des plantes dicotylédonées et monocotylédonées. Ladite invention a également trait à des vecteurs comprenant des zones régulatrices liées de manière fonctionnelle à un gène hétérologue d'intérêt, ainsi qu'à des cultures cellulaires végétales et à des plantes transgéniques contenant ces vecteurs. En outre, l'invention porte sur un procédé de préparation d'une plante à l'aide des régions régulatrices de cette invention.

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