Release reagent for vitamin d compounds

G - Physics – 01 – N

Patent

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G01N 33/82 (2006.01)

Patent

CA 2624129

The present invention concerns a reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein, a method for the detection of a 25-hydroxyvitamin D compound in which the 25-hydroxyvitamin D compound is released from vitamin D-binding protein using this reagent and the mixture obtained in this manner is analysed, the use of the reagent to release vitamin D compounds as well as a kit for detecting 25-hydroxyvitamin D which contains the reagent for releasing vitamin D compounds in addition to the usual immunological reagents.

La présente invention concerne une composition réactive pour la libération de composés de vitamine D liés à la protéine liant la vitamine D ainsi qu~un procédé de détection d~un composé de 25-hydroxyvitamine D. Dans ce procédé, le composé 25-hydroxyvitamine D est libéré à partir de la protéine liant la vitamine D en utilisant ce réactif et le mélange ainsi obtenu est analysé. L~invention concerne également l~utilisation du réactif visant à libérer des composés de vitamine D ainsi qu~un kit de détection de la 25-hydroxyvitamine D qui contient, outre les réactifs immunologiques habituels, le réactif de libération de composés de vitamine D.

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