Removal of protein a from antibody preparations

Details Removal of protein a from antibody preparations Removal of protein a from antibody preparations

C - Chemistry, Metallurgy

07

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167/139, 167/44,

C07K 1/18 (2006.01) C07K 16/00 (2006.01) C07K 16/06 (2006.01) C07K 16/36 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1339188

Protein A is selectively isolated from an antibody - Protein A mixture by exposing the mixture to an anion exchange material under conditions sufficient to adsorb both components and then sequentially eluting the antibodies and protein A under conditions of increasing ionic strength. Resulting antibody preparations have less than about 15 ng of Protein A per mg of antibody.

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