Renaturation and activation of the proteinase k zmyogen...

Details Renaturation and activation of the proteinase k zmyogen... Renaturation and activation of the proteinase k zmyogen...

C - Chemistry, Metallurgy

12

N

C12N 9/58 (2006.01) C07K 1/113 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2435753

The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.

L'invention concerne une protéinase K recombinante et son procédé de production. Ce procédé consiste: a) à transformer une cellule hôte par un acide nucléique recombinant qui codant pour le précurseur zymogène de la protéinase K, b) à cultiver la cellule hôte de telle manière que le précurseur zymogène de la protéinase K apparaisse sous la forme de "corps d'inclusion" dans la cellule hôte, c) à isoler les "corps d'inclusion" et à les renaturer dans des conditions telles que la partie protéinase du précurseur zymogène apparaisse dans sa conformation naturelle, d) à activer et à purifier la protéinase K renaturée.

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