Renilla reniformis green fluorescent protein and mutants...

Details Renilla reniformis green fluorescent protein and mutants... Renilla reniformis green fluorescent protein and mutants...

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C12N 1/36 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2401544

The invention relates to recombinant polynucleotides encoding the Green Fluorescent Protein (GFP) from R. reniformis, as well as polynucleotides encoding variants and fusion polypeptides of R. reniformis GFP. The invention further relates to vectors encoding R. Reniformis GFP and variants and fusions thereof, as well as to cells comprising and/or expressing such vectors. The invention also relates to recombinant R. reniformis GFP polypeptides and fusion polypeptides and variants thereof, as well as to methods of making and using such polypeptides both in vivo and in vitro.

L'invention concerne des polynucléotides de recombinaison codant pour la protéine verte fluorescente (GFP) de R. reniformis, ainsi que des polynucléotides codant pour des variants et des polypeptides de fusion de la GFP de R. reniformis. L'invention concerne également des vecteurs codant pour la GFP deR. Reniformis, des variants et des fusions, ainsi que des cellules comprenant et/ou exprimant ces vecteurs. L'invention concerne en outre des polypeptides de recombinaison de la GFP deR. reniformis, des polypeptides de fusion et des variants, ainsi que des méthodes permettant d'obtenir et d'utiliser ces polypeptides in vivo et in vitro.

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