Repertoire of allo-restricted peptide-specific t cell...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) C07K 14/705 (2006.01) C07K 14/725 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2751762

The present invention is directed to a kit-of-parts or composition containing nucleic acid sequences coding for high-avidity, allo-restricted TCR, wherein the TCR are independently directed against the tyrosinase antigen, the melan-A antigen and the survivin antigen. The invention is further directed to a kit-of-parts or composition containing at least three groups of transgenic lymphocytes transformed with vectors coding for TCR against said antigens. Furthermore, the present invention provides a pharmaceutical composition and its use in the treatment of diseases involving malignant cells expressing said tumor-associated antigens. The invention further relates to a nucleic acid molecule coding for a TCR that recognizes the survivin antigen, a TCR encoded thereby and a T cell expressing said TCR. Further, the invention discloses a vector, a cell and a pharmaceutical composition encoding/containing same and their use in the treatment of diseases involving malignant cells expressing survivin.

La présente invention porte sur un kit de composants ou une composition contenant des séquences d'acide nucléique codant pour des TCR allo-restreints d'avidité élevée, les TCR étant indépendamment dirigés contre l'antigène tyrosinase, l'antigène melan-A et l'antigène survivine. L'invention porte en outre sur un kit de composants ou une composition contenant au moins trois groupes de lymphocytes transgéniques transformés avec des vecteurs codant pour des TCR dirigés contre lesdits antigènes. En outre, la présente invention porte sur une composition pharmaceutique et sur son utilisation pour le traitement de maladies mettant en jeu des cellules malignes exprimant lesdits antigènes associés à des tumeurs. L'invention porte en outre sur une molécule d'acide nucléique codant pour un TCR qui reconnaît l'antigène survivine, sur un TCR codé par celle-ci et sur un lymphocyte T exprimant ledit TCR. En outre, l'invention porte sur un vecteur, une cellule et une composition pharmaceutique codant/contenant ceux-ci et sur leur utilisation pour le traitement de maladies mettant en jeu des cellules malignes exprimant la survivine.

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