Replica amplification of nucleic acid arrays

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2305449

A method of producing a plurality of a nucleic acid array, comprising, in order, the steps of amplifying in situ nucleic acid molecules of a first randomly-patterned, immobilized nucleic acid array comprising a heterogeneous pool of nucleic acid molecules affixed to a support, transferring at least a subset of the nucleic acid molecules produced by such amplifying to a second support, and affixing the subset so transferred to the second support to form a second randomly-patterned, immobilized nucleic acid array, wherein the nucleic acid molecules of the second array occupy positions that correspond to those of the nucleic acid molecules form which they were amplified on the first array, so that the first array serves as a template to produce a plurality, is disclosed.

L'invention porte sur un procédé d'obtention d'une série de réseaux d'acide nucléique comprenant dans l'ordre les étapes suivantes: amplification in situ de molécules d'acide nucléique d'un premier réseau d'acide nucléique à séquence aléatoire et immobilisé comprenant un pool hétérogène de molécules d'acide nucléique fixés à un support; transfert sur un deuxième support d'au moins un sous-ensemble de molécules d'acide nucléique produites par ladite amplification; et fixation du sous-ensemble ainsi transféré sur le deuxième support de manière à former un deuxième réseau d'acide nucléique à séquence aléatoire et immobilisé dans lequel les molécules du deuxième réseau occupent des positions correspondant à celles des molécules à partir desquelles elles ont été amplifiées sur le premier réseau, de manière à ce que le premier réseau serve de gabarit pour en produire plusieurs.

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