Replication assay for detecting anti-viral substances

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/70 (2006.01) C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2431820

The invention relates to a replication assay for detecting anti-virally active inhibitors in the form of a single-well assay. Said assay consists of a combination of a natural virus isolation agent, suspended target cells and a cell culture medium containing foetal calf serum, whereby the concentration of the foetal calf serum is less than 5 % by volume.

L'invention concerne un test de réplication destiné à la recherche d'inhibiteurs antiviraux efficaces. Ce test monotope comprend la combinaison d'un isolat viral naturel, de cellules cibles en suspension et d'un milieu de culture cellulaire contenant du sérum de veau foetal dont la concentration est inférieure à 5 % du volume.

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