Reporter cell line system for detecting cytomegalovirus and...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/70 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2250967

Cell lines and methods for detecting cytomegalovirus infection and identifying modulators of cytomegalovirus gene expression are disclosed. The cell lines carry an integrated plasmid that contains a reporter gene under the control of a cytomegalovirus promoter. Such cell lines may be used, for example, for specifically identifying HCMV infection in a biological sample.

Lignées cellulaires et méthodes de détection de l'infection par le cytomégalovirus et d'identification des modulateurs de l'expression génique du cytomégalovirus. Les lignées cellulaires portent un plasmide intégré contenant un gène marqueur commandé par un promoteur du cytomégalovirus. Ces lignées cellulaires peuvent être utilisées, par exemple, pour identifier spécifiquement une infection par le cytomégalovirus humain dans un prélèvement biologique.

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