C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 1/04 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2442521
The present invention provides plant selection strategies to identify and select plants cells, tissue or entire plants which comprise a coding region of interest. The plant selection strategy of the present invention generally involves i) transforming the plant, or portion thereof with a first nucleotide sequence comprising a first regulatory region in operative association with a first gene, and an operator sequence, the first gene encoding a tag protein; ii) screening for the transformed plant; iii) introducing a second nucleotide sequence into the transformed plant, or portion thereof to produce a dual transgenic plant, the second nucleotide sequence comprising a second regulatory region, in operative association with a second gene, and a third regulatory region in operative association with a third gene, the second gene comprising a coding region of interest, the third gene encoding a repressor capable of binding to the operator sequence thereby inhibiting expression of the first gene, and; iv) selecting for the dual transgenic plant by identifying plants, or portions thereof deficient in the tag protein, or an identifiable genotype or phenotype associated therewith. The first gene may be a conditionally lethal gene and the tag protein may be a conditionally lethal protein.
Bate Nicholas
Hannoufa Abdelali
Hegedus Dwayne
Lydiate Derek
Gowling Lafleur Henderson Llp
Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food
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