Rescue of canine distemper virus from cdna

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/40 (2006.01) C07K 14/12 (2006.01) C07K 14/13 (2006.01) C12N 7/00 (2006.01)

Patent

CA 2412621

This invention relates to a method for recombinantly producing via rescue canine distemper virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the canine distemper virus as an attenuated and/or infectious viruses. The recombinant viruses can be prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide/polynucleotide deletions, insertions, substitutions and rearrangements, in the genome can be obtained.

Cette invention porte sur un procédé de production par recombinaison, via la technique du sauvetage du virus de la maladie de Carré, d'un virus d'ARN monobrin, sens négatif, non segmenté, et sur des compositions immunogènes obtenues. Selon d'autres réalisation, l'invention porte sur des procédés de production du virus de la maladie de Carré sous forme d'un virus atténué et/ou infectieux. Les virus recombinés peuvent être préparés à partir de clones d'ADNc et, en conséquence, il est possible d'obtenir des virus présentant des modifications définies comprenant des délétions nucléotidiques/polynucléotidiques, des insertions, des substitutions et des transpositions, dans le génome.

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