Rescue of influenza virus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2592439

The invention relates to the field of influenza vaccine production. Influenza vaccines have been produced in embryonated hens'eggs for over 50 years, but recently there have been considerable efforts to develop cell culture systems for vaccine production. The invention provides a nucleic acid comprising an influenza gene segment and a bacteriophage polymerase promotor or a complementary strand of said nucleic acid, and a cell comprising such a nucleic acid capable of producing desired influenza virus. Furthermore, the invention provides a composition comprising a cell or material derived from a cell according to the invention and a virus or material derived from a viral particle according to the invention.

L'invention se rapporte au domaine de la production de vaccins antigrippaux. Cela fait plus de 50 ans que des vaccins antigrippaux sont produits dans des oeufs de poule embryonnés, mais, récemment, des efforts considérables ont été déployés pour développer des systèmes de culture cellulaire pour la production de vaccins. Cette invention concerne un acide nucléique, comprenant un segment de gène grippal et un promoteur de polymérase de bactériophage ou un brin complémentaire dudit acide nucléique, et une cellule comprenant un tel acide nucléique capable de produire le virus grippal souhaité. De plus, ladite invention concerne une composition comprenant une cellule ou une substance dérivée d'une cellule selon l'invention et un virus ou une substance dérivée d'une particule virale selon l'invention.

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