Resolvase-catalysed, sequence-specific dna-recombination in...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01) C07K 14/245 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/22 (2006.01) C12N 15/52 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2394827

The invention relates to a method for the sequence-specific recombination of DNA in eukaryotic cells, comprising the following steps: introducing a first DNA sequence which contains a res sequence into a cell, introducing a copy of the first DNA sequence and performing the sequence-specific recombination using the influence of a resolvase or its derivative. The invention also relates to a method for testing topoisomerase inhibitors in a eukaryotic cell, comprising the following steps: introducing a first DNA sequence according to SEQ ID NO:1 into a cell, introducing a copy of the first DNA sequence according to SEQ ID NO:1 in direct orientation, introducing a wild-type resolvase and adding the topoisomerase inhibitors which are to be tested.

L'invention concerne un procédé de recombinaison avec une séquence spécifique d'ADN dans des cellules eucaryotes comprenant l'introduction dans une cellule d'une première séquence d'ADN comprenant elle-même une séquence res, l'introduction d'une copie de la première séquence d'ADN et la réalisation de la recombinaison avec une séquence spécifique au moyen d'une résolvase ou d'un dérivé de celle-ci. L'invention concerne également un procédé pour tester des inhibiteurs de topoisomérase dans une cellule eucaryote, comprenant l'introduction dans une cellule d'une première séquence d'ADN conforme à SEQ ID NO:1, l'introduction dans une cellule d'une copie de la première séquence d'ADN conforme à SEQ ID NO:1 en orientation directe, l'introduction d'une résolvase de type sauvage et l'adjonction des inhibiteurs de topoisomérase à tester.

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