Restriction amplification assay

Details Restriction amplification assay Restriction amplification assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2122734

2122734 9310264 PCTABS00022 The present invention relates to a method, reagent and kit for the the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.

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