C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) A61K 35/12 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C07K 14/705 (2006.01) C12N 15/867 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2146929
This invention provides a mammalian retroviral producer cell constructed by transfecting a retroviral packaging cell with a retroviral vector containing the human multiple drug resistance gene. The mammalian retroviral producer cell produces retroviral particles suitable for tranducing target cells. Accordingly, the producer cell of this invention can be used to transduce target mammalian cell with the human MDR gene, and with a second, non-selectable gene, e.g. insulin, .beta.-globin or a major histocom- patibility gene. The producer cell of this invention can be used in methods of treating a mammal afflicted with a cancer and of treating a mammal afflicted with a disorder characterized by abnormal expression of a non-selectable gene which involve trans- ducing suitable cells from the mammal with the human MDR gene and then selecting with an MDR-responsive drug for cells which express the MDR gene. This producer line is demonstrated to be safe and free of replication-competent retrovirus in con- trast to the producer lines.
Bank Arthur
Goff Stephen P.
Ward Maureen
Gowling Lafleur Henderson Llp
The Trustees Of Columbia University
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