Retroviral vectors pseudotyped with srv-3 envelope...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01)

Patent

CA 2234219

A pseudotype vector virus particle, comprising (1) as viral RNA in the particle, a recombinant vector comprising a retroviral LTR and packaging signal regions and further comprising a coding region encoding a non- retroviral protein or genetic control sequence located downstream of the packaging signal or encoding a heterologous retroviral sequence useful in treatment of disease, such as an HIV protein sequence; (2) as capside proteins, non-SRV capsid proteins; and (3) as envelope glycoproteins, SRV envelope glycoproteins. The invention also includes packaging cell lines for production of these pseudotyped retroviral vector particles.

L'invention porte sur une particule à vecteur viral pseudotypé, comprenant: 1) comme ARN viral dans la particule, un vecteur recombiné qui comporte un LTR rétroviral et des régions à signal d'encapsidation, plus une région de codage codant une protéine non-rétrovirale ou une séquence de contrôle génétique située en aval du signal d'encapsidation, ou codant une séquence rétrovirale hétérologue utile pour le traitement d'une maladie, telle qu'une séquence de protéine VIH; 2) comme protéines capsides, des protéines capsides non SRV; et 3) comme glycoproteines enveloppes, des glycoprotéines enveloppes SRV. L'invention comprend également des lignées cellullaires d'encapsidation servant à produire ces particules à vecteur rétroviral pseudotypé.

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