Retrovirus producting cells utilizing a high multiplicity of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) A61K 48/00 (2006.01) C07K 14/145 (2006.01) C07K 14/15 (2006.01) C12N 5/10 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2365655

Retroviral vector particle producing cells are provided, wherein the cell (a) has greater than 5 stably integrated copies of a retroviral vector construct; (b) produces greater than 10 infectious recombinant retroviral vector particles per cell per day; and (c) produces replication incompetent retroviral vector particles.

L'invention concerne des cellules produisant des particules de vecteur rétroviral, lesquelles cellules (a) comprennent plus de 5 copies stablement intégrées d'une construction de vecteur rétroviral; (b) produisent plus de 10 particules infectieuses de recombinaison de vecteur rétroviral par cellule et par jour; et (c) produit des particules de vecteur rétroviral incapables de se répliquer.

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