Reverse breeding

A - Human Necessities – 01 – H

Patent

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A01H 1/04 (2006.01) A01H 1/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2454609

The present invention relates to a method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes; wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.

L'invention concerne une méthode efficace de fabrication d'organismes homozygotes à partir d'un organisme de départ non humain hétérozygote. Cette méthode consiste à fournir un organisme de départ hétérozygote, à permettre à cet organisme de départ de fabriquer des cellules haploïdes, à créer des organismes homozygotes à partir des cellules haploïdes ainsi obtenues, et à sélectionner les organismes présentant l'ensemble désiré de chromosomes. Au cours de la fabrication de cellules haploïdes, aucune recombinaison ne peut se produire afin d'obtenir un nombre limité de cellules haploïdes génétiquement différentes. La recombinaison peut également être évitée ou supprimée.

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