Reverse sample genome probing of sulphate-reducing bacteria

Details Reverse sample genome probing of sulphate-reducing bacteria Reverse sample genome probing of sulphate-reducing bacteria

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2042293

A reverse genome probe process for detecting the presence of sulfate-reducing bacteria consists of: (a) obtaining an individual culture of at least one "selected standard" (which selected standard may be either a single sulfate-reducing bacterium or a group of more than one sulfate-reducing bacteria having homologous, cross-hybridizing genomes); (b) affixing a nucleic acid preparation from each selected standard to a solid surface; (c) preparing a "reverse" probe by labeling a nucleic acid preparation obtained from a sample taken from an oil field environment; (d) contacting the reverse probe with the solid surface under conditions which permit hybridization; and (e) detecting the presence of any reverse genome probe. By using the "reverse" probe (i.e. a probe prepared from the sample, as opposed to a probe prepared from a selected microorganism), a single incubation may be used for the initial characterization of an unknown sample.

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